摘要：Mr. Bao did not finish in time again, he has broken the fouth deadline he set together with the bosses.i kept silent for more than 1 month and was waiting the desetion of my bossesfight back or hold s... 阅读全文

摘要：For this month, we went over the process what we did last half year and made clear what the relations were between contigs or between contigs and the reference... last week,,,i designed more than 300... 阅读全文

摘要：Mr Bao knows a little about this thing, furthernmore thinks high of himself, one of my coworks will leave for Beijing, Mr Bao thinks it more than ours... cheat money and be shamealways, why did thi... 阅读全文

摘要：when Mr Bao was here, we held togther and had more than 7 hours meeting... i am sure that somebody wanted to quit and find a new way forwards. after tough neogtiation we held on and kept the former o... 阅读全文

摘要：another sunny day i arrive hangzhuo... another overnight i arrive here... another promise i get and arrive here... a big chellenge we face-- somebody wanna quit, somebody wanna to take control and ga... 阅读全文

摘要：i got information that the leader fired many employees and would reduce 25% salary ... here gets worse and worse... 阅读全文

摘要：the pOX1 (discovered in Ba)sequence is not the key role for assembling that plasmid... maybe i will find another... 阅读全文

摘要：We are now designing the primers between lines and preparing to PCR on the BAC and chromosome DNA... i think those lines will be contigs in the near future... 阅读全文

摘要：the contigs which have paired reads relatioan cluster into a line... we are just making them right and filling gapsbetween sontigsin different lines. we get the line statistic number---159,maybe ... 阅读全文

摘要：some person picked the wrong templates so, we had sequenced the vector for many times... i kept working for several days and made right ... it sounds stupid but it happended indeed be patient and h... 阅读全文

摘要：This afternoon Mr Bao told me that Mr Yu telephoned him yesterday in US... Mr Yu wanna do DNA chip experiencestelling more than 1000 different strains (Bt)... It is obverse to see that Mr Yu were... 阅读全文

摘要：Mr. Xie gave me that web page, and thanks to him http://hrst.mit.edu/hrs/bioinformatics/public/PrimarySite.html http://hrst.mit.edu/hrs/bioinformatics/public/SecondarySite.html from MIT i love v... 阅读全文

摘要：We number the whole number of blocks and it is 301 or so almost they are all chromosome DNA... maybe in the end of January we will get 300 or so contigs... But it will still be a disaster to order th... 阅读全文

摘要：I am now doing the BAC(the larger alien DNA bank) position work, and i found that many pairs supported a larger possible block which looked not like chromosome DNA... First Blast job i find that it i... 阅读全文

摘要：Bacillus' genome is in the high AT content: GC content is 30% or so, what a disaster that we do many experients in 37 degree, that would hurt that DNA of Bt a lot... my God... 阅读全文

摘要：1> all the stuff and work here is just an ok to be considered, and we got two piles ofPCR result in large quantity, which reduces the number of contigs below to 1000 already. 2>another PCR is ... 阅读全文

摘要：Dear Sir I have already told you the schedule about our work in this genomic institute. And I have another something as follow to tell you. 1. The difficulty and complexity, which we are e... 阅读全文