ERROR: Paired-end reads were detected in single-end read library : SRR208975

 

001、featureCount命令报错：ERROR: Paired-end reads were detected in single-end read library : SRR208975

featureCounts -a GCF_034140825.1_ASM3414082v1_genomic.gtf -o sample_name_gene_counts.txt sample_name.bam -T 16 -g gene_id

 

002、解决方法：增加-p选项

featureCounts -p -a GCF_034140825.1_ASM3414082v1_genomic.gtf -o sample_name_gene_counts.txt sample_name.bam -T 16 -g gene_id

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