fastq-dump quit with error code 3

 

001、sra数据转换fastq数据报错如下:

 

002、报错产生的原因“:

没有网络连接。

 

003、验证

a、无网络连接

[root@PC1 test1]# ls
SRR11076280
[root@PC1 test1]# ping -c 3 www.baidu.com                        ## 检测网络连通性
ping: www.baidu.com: Name or service not known
[root@PC1 test1]# fastq-dump --split-files SRR11076280           ##  将sra数据转换为fastq
2024-01-18T12:49:09 fastq-dump.3.0.10 warn: directory not found while opening manager within virtual file system module - can't open CM004562.1 as a RefSeq or as a WGS
2024-01-18T12:49:09 fastq-dump.3.0.10 err: directory not found while opening manager within virtual file system module - failed SRR11076280

=============================================================
An error occurred during processing.
A report was generated into the file '/root/ncbi_error_report.txt'.
If the problem persists, you may consider sending the file
to 'sra-tools@ncbi.nlm.nih.gov' for assistance.
=============================================================

fastq-dump quit with error code 3

 

b、有网络连接

[root@PC1 test1]#  ls
SRR11076280
[root@PC1 test1]# ping -c 3 www.baidu.com                  ## 网络连通性
PING www.a.shifen.com (110.242.68.3) 56(84) bytes of data.
64 bytes from 110.242.68.3 (110.242.68.3): icmp_seq=1 ttl=128 time=13.8 ms
64 bytes from 110.242.68.3 (110.242.68.3): icmp_seq=2 ttl=128 time=17.4 ms
64 bytes from 110.242.68.3 (110.242.68.3): icmp_seq=3 ttl=128 time=15.6 ms

--- www.a.shifen.com ping statistics ---
3 packets transmitted, 3 received, 0% packet loss, time 2003ms
rtt min/avg/max/mdev = 13.843/15.672/17.495/1.490 ms
[root@PC1 test1]# fastq-dump --split-files SRR11076280        ## 转换为fastq,可以正确转换
^C[root@PC1 test1]# ls
SRR11076280  SRR11076280_1.fastq  SRR11076280_2.fastq
[root@PC1 test1]# ll -h
total 6.3G
-rw-r--r--. 1 root root 6.2G Jan 18 20:40 SRR11076280
-rw-r--r--. 1 root root  39M Jan 18 20:53 SRR11076280_1.fastq
-rw-r--r--. 1 root root  39M Jan 18 20:53 SRR11076280_2.fastq

 

 

posted @ 2024-01-18 12:54  小鲨鱼2018  阅读(2141)  评论(0)    收藏  举报