【文献导读】--AD

文献：A genome-wide gene-expression analysis and database in transgenic mice during developmentof amyloid or tau pathology，

　　提供了4个品系的小鼠的MicroArray数据，并进行了qPCR验证，并与GWAS的数据进行了比较。

　　results：

　　　　免疫相关的基因的表达与plaque相关，突触相关的基因与神经纤维缠结相关。 Immune gene expression correlated tightly with plaques whereas synaptic genes correlated negatively with neurofibrillary tangles. 

　　data：

　　　　可下载使用。　　This Resource opens up many areas for investigation. All data are available and searchable at http://www.mouseac.org.

  

文献：Neuroinflammation — using big data to inform clinical practice，2016

 　　高通量的数据有：

　　　　‘omics’ data：

　　　　other type of data: imaging、epidemiology、lifestyle、behavioural, clinical and paraclinical information

 

文献：Altered bile acid profile in mild cognitive impairment and Alzheimer's disease: Relationship to neuroimaging and CSF biomarkers.2019.02

　　血液标志物

 

文献： Short and long TNF‐alpha exposure recapitulates canonical astrogliosis events in human‐induced pluripotent stem cells‐derived astrocytes, Glia (2020)

　　样本：自愿捐献者尿液中发现的人类细胞进行重编程，将其转变为多能干细胞 

　　中文导读：http://news.bioon.com/article/6750536.html　　

 

文献：Quantitative proteomics of acutely-isolated mouse microglia identifies novel immune Alzheimer’s disease-related proteins，2018，Mol Neurodegener

　　对三种小鼠——control、急性炎症模型小鼠LPS、慢性炎症小鼠(AD的5xFAD）的小胶质细胞，进行质谱分析，发现了187个差异表达蛋白（如：Apoe, Clu，Htra1，Cotl1 and Hexb）。这篇文章首次描绘了神经退行性疾病的小胶质细胞的蛋白图谱。

 

文献：Microglia isolation from adult mouse brain，2014，Methods Mol Biol.

　　Although microglia isolation from embryonic or postnatal mouse brain is possible using a number of different protocols, microglia isolation from adult brain is more challenging and often results in low yields. Here, we describe a protocol to isolate intact microglia from adult mouse brain for functional assays, 

　　adult mouse的小胶质细胞的分离是很困难的，而且产出量很低。

 

文献：Molecular Signatures of the Aging Brain: Finding the Links Between Genes and Phenotypes.2019，Neurotherapeutics 

　　后续可以看。

 

文献：Cerebellar Contribution to Motor and Non-motor Functions in Parkinson's Disease: A Meta-Analysis of fMRI Findings.2020，FrontNeuro

　　搜索关键词：non-motor symptoms Parkinson genetic marker 

 

文献：Somatic variants in new candidate genes identified in focal cortical dysplasia type II，2020，epilesia

一种儿童、耐药型癫痫。17个样本。WES测序。