fastp 使用

fastp -i  R1.fq.gz -I R2.fq.gz -o R1.clean.fastq -O R2.clean.fastq

--json fastp.json --html fastp.html

--qualified_quality_phred 15

--unqualified_percent_limit 40 --length_required 25

--detect_adapter_for_pe --cut_tail --cut_window_size 5

--cut_mean_quality 20 --detect_adapter_for_pe

--overrepresentation_analysis --thread 16