摘要: In this context, ‘local’ means you are running BLAST on your own server, not at NCBI or anyone else’s server. This gives you the flexibility of compar... 阅读全文
posted @ 2014-10-16 22:59 skylinelzy 阅读(419) 评论(0) 推荐(0)
摘要: The bedtools utilities are a swiss-army knife of tools for a wide-range of genomics analysis tasks. The most widely-used tools enable genome arithmeti... 阅读全文
posted @ 2014-08-15 15:07 skylinelzy 阅读(405) 评论(0) 推荐(0)
摘要: Some time when you want to change the fasta seq into one lineFor awk: awk '/^>/&&NR>1{print "";}{ printf "%s",/^>/ ? $0" ":$0 }' YourFile > newfilenam... 阅读全文
posted @ 2014-08-12 18:14 skylinelzy 阅读(3838) 评论(0) 推荐(0)
摘要: Tablet is a lightweight, high-performance graphical viewer for next-generation sequence assemblies and alignmentsDownload Tablet : Information & Compu... 阅读全文
posted @ 2014-08-11 10:27 skylinelzy 阅读(255) 评论(0) 推荐(0)
摘要: 数字PCR即Digital PCR,它是是一种核酸分子绝对定量技术。数字PCR可让你能够直接数出DNA分子的个数,是对起始样品的绝对定量。 在定量PCR时,我们常常纠结一个问题,究竟是相对定量还是绝对定量呢?现在我们再也不需要去纠结这个问题了,因为数字PCR帮我们解决了。尽管这两种技术有些类似,都... 阅读全文
posted @ 2014-06-11 12:10 skylinelzy 阅读(4316) 评论(0) 推荐(0)