免疫细胞浸润百分比堆积条形图
该条形图展示的是每个TCGA的肿瘤样本中各个免疫细胞所占的比例。
输入数据
该输入数据的每一行显示的是一个TCGA的肿瘤样本中各个免疫细胞所占的比例。每一行的数据用制表符“\t”分隔。
Mixture B cells naive B cells memory Plasma cells T cells CD8 T cells CD4 naive T cells CD4 memory resting T cells CD4 memory activated T cells follicular helper T cells regulatory (Tregs) T cells gamma delta NK cells resting NK cells activated Monocytes Macrophages M0 Macrophages M1 Macrophages M2 Dendritic cells resting Dendritic cells activated Mast cells resting Mast cells activated Eosinophils Neutrophils TCGA-VQ-AA6J-01A-11R-A414-31 0.122186674 0 0.015362249 0.028902924 0 0.160768937 0.02591284 0.068611584 0.033803949 0 0 0.012525355 0 0.363159742 0.079920517 0.05303078 0 0 0.03581445 0 0 0 TCGA-VQ-A91D-01A-11R-A414-31 0.051348636 0 0.008172379 0.079469663 0 0.106860657 0.008181623 0.067017808 0.007933433 0 0.018787382 0.014297553 0 0.201468786 0.146233333 0.090970146 0 0.011314463 0 0.177945331 0 0.009998807 TCGA-BR-8372-01A-11R-2343-13 0.001760746 0 0 0.205358003 0 0.095136351 0.176166502 0.045423354 0.000448383 0 0 0 0.003422254 0.107769841 0.10016814 0.15513019 0.06212412 0 0.045272717 0 0.001819399 0 TCGA-CD-8533-01A-11R-2343-13 0.222226465 0 0.074093646 0.056621402 0 0.15357101 0.066436865 0.008194517 0.04139503 0 0.006790187 0 0 0.04443435 0.043483231 0.134173183 0.105263314 0 0.035456107 0 0.007860694 0 TCGA-D7-A747-01A-22R-A33Y-31 0.247949248 0.018800323 0 0.113533785 0 0.271232918 0 0.014815118 0.072346893 0 0 0.056186396 0.003899288 0.011852412 0.02326777 0.087803243 0.000785244 0.016085265 0.061442099 0 0 0 TCGA-VQ-A91A-01A-11R-A414-31 0.052137546 0 0.036903843 0.006694379 0 0.171274942 0 0 0 0 0.034712848 0 0.008094716 0.285056226 0.001897999 0.136190623 0 0.092300144 0 0.140810603 0 0.033926131 TCGA-BR-4369-01A-01R-1157-13 0.10936253 0 0.11347573 0.059926682 0 0.293117987 0.036330889 0.026421797 0.026265638 0 0.012457433 0 0.00696376 0.013747272 0.044921235 0.104549958 0.128025349 0 0.006806311 0 0.017627428 0 TCGA-D7-8573-01A-11R-2343-13 0.165823699 0 0.004822539 0.072208659 0 0.299217188 0.027634768 0.0320419 0 0 0 0 0.017265037 0.185518405 0.119800333 0 0.001681564 0 0 0.073985908 0 0 TCGA-CG-4437-01A-01R-1802-13 0.078790998 0 0.011327467 0.239965536 0 0.04508004 0.139122848 0.044792483 0.019054694 0 0.021311699 0.008169418 0.008088004 0.02501395 0.073351587 0.199063908 0.016257766 0.001306321 0.018469968 0.00235924 0 0.048474073 TCGA-BR-8589-01A-11R-2402-13 0.057505139 0 0.00369182 0.409028906 0 0.007029469 0.113797087 0.059387187 0.014546917 0 0 0.029278637 0.007497186 0.010500819 0.078373304 0.096100405 0.077893022 0.004981631 0.030388472 0 0 0 TCGA-B7-A5TK-01A-12R-A36D-31 0.022183833 0 0.008160488 0.353934688 0 0 0.25779089 0.010143417 0.016760667 0 0 0.013774982 0.005814627 0.015632038 0.123524886 0.088090249 0.013708865 0.023626314 0.042656412 0 0 0.004197644 TCGA-RD-A8N9-01A-12R-A39E-31 0.10146504 0 0.065819039 0.117775355 0 0.193073083 0.039595427 0.072723603 0.100709815 0 0 0.004287062 0.002807689 0.068930903 0.053371747 0.108774713 0.020976298 0 0.049690224 0 0 0 TCGA-IN-AB1X-01A-11R-A39E-31 0.130278869 0 0.026116809 0.298302942 0 0.161933674 0.072912887 0.027247527 0.05012481 0 0.036103455 0 0.017815909 0.052247687 0.0172014 0.032435398 0 0.055724838 0 0.014460202 0 0.007093592 TCGA-CG-4305-01A-01R-1157-13 0.089648269 0 0.002330765 0.108883503 0 0.173374239 0.147591926 0.008444609 0.004553349 0.024344737 0.026535541 0 0.009347313 0.03937453 0.112693405 0.209101435 0.007486141 0 0.03350219 0 0 0.002788048 TCGA-FP-8211-01A-11R-2343-13 0.179926876 0 0.038618079 0.160118554 0 0.297476507 0.053158847 0.02012318 0.07749334 0 0 0.029895302 0 0.046043737 0.027138725 0.044703147 0 0.016168232 0.009135473 0 0 0 TCGA-VQ-A94P-01A-13R-A414-31 0.115919157 0 0.034204227 0.114596329 0 0.332906217 0.012998417 0.019487143 0.018545853 0 0 0.035576351 0.0422579 0.012001789 0.075433374 0.116386276 0.01136735 0 0.058319616 0 0 0 TCGA-CD-5803-01A-11R-1602-13 0.064142947 0 0.002622022 0.246608875 0 0.226319024 0.059590178 0.010769439 0.104387941 0 0 0 0.008962578 0.032778038 0.075586456 0.081639893 0.05495889 0.012384076 0.019249642 0 0 0 TCGA-VQ-A8P3-01A-11R-A36D-31 0.08974966 0 0.01479577 0.304495202 0 0.206967587 0.097826453 0.009610597 0.052224945 0 0 0.032307389 0.010466407 0.039236254 0.039470545 0.035883114 0.019878187 0 0.047087891 0 0 0 TCGA-SW-A7EB-01A-11R-A354-31 0.218833276 0 0.057381411 0.120617652 0 0.202848472 0 0.101598705 0.054827252 0 0 0.063049842 0.007342783 0.055544027 0.051633469 0.034787333 0.001527897 0 0.030007879 0 0 0 TCGA-BR-6564-01A-12R-1884-13 0.108747654 0 0 0.186669102 0 0.233534116 0.004481513 0 0.078232518 0 0 0.009119715 0.015018501 0 0.08962081 0.129773137 0.016552859 0 0.128250075 0 0 0 TCGA-VQ-A924-01A-11R-A414-31 0.009949809 0 0.000889406 0.155885409 0 0.178206749 0.147919727 0.048993372 0.010617517 0.003066854 0.001198034 0 0 0.156979277 0.093224495 0.148979497 0 0.023783678 0.017624263 0 0 0.002681913 TCGA-HU-A4GY-01A-21R-A24K-31 0.088518831 0.142380361 0 0.103198696 0 0.244396963 0.028745877 0.024089024 0.058979102 0 0 0 0.000113317 0.087789462 0.05159298 0.134086728 0.010926503 0.005553669 0.019628488 0 0 0 TCGA-HF-7134-01A-11R-2055-13 0.043544985 0 0.148526946 0.044733707 0 0.23774073 0.010647524 0.001215128 0.045066958 0 0.04511407 0 0 0.183107849 0.031359813 0.10793338 0 0.026734849 0 0.039354311 0 0.034919751 TCGA-VQ-AA6K-01A-11R-A414-31 0.126295206 0 0.133103624 0.164978755 0 0.223656709 0.094679539 0.013102401 0.016294103 0 0.000272463 0.006629604 0.002877496 0.064079007 0.035470683 0.065487247 0.02682365 0.003778563 0.021845959 0.00062499 0 0 TCGA-BR-A4J4-01A-12R-A251-31 0.102156244 0 0 0.024447341 0 0.324116026 0.017616805 0.002060512 0.079882035 0.000584266 0 0.019146527 0.003556257 0.004168963 0.143920359 0.101366489 0.159859881 0 0 0.017118294 0 0 TCGA-IN-A7NT-01A-21R-A354-31 0.007514663 0 0.000791329 0.058401474 0 0 0 0.042354433 0.060934904 0 0 0.003233991 0 0.231445791 0.022986965 0.093091693 0 0.187181189 0 0.278926536 0 0.013137032 TCGA-RD-A8N2-01A-12R-A36D-31 0.100701945 0 0 0.17173349 0 0.235788538 0.0255386 0.000735955 0.033823056 0 0 0.015160596 0.020829314 0 0.026778174 0.213370242 0.052805552 0.030045615 0.072688925 0 0 0 TCGA-RD-A8N1-01A-12R-A36D-31 0.117539357 0.006028618 0.048564005 0.116203418 0 0.239565335 0.037011081 0.02447297 0.107494919 0 0 0.011283044 0 0.126087122 0.053510299 0.079309942 0.017148552 0.005492219 0.010289118 0 0 0 TCGA-MX-A5UJ-01A-11R-A31P-31 0.136427253 0 0.004681179 0.113896485 0 0.335867159 0.004148929 0.056166075 0.008926196 0 0 0.008041271 0.00424248 0.038580293 0.088475283 0.100694848 0 0.009302259 0.037844942 0.037302279 0 0.015403068 TCGA-FP-A4BF-01A-12R-A36D-31 0.028755807 0 0.053690302 0.173848064 0 0.209787324 0.154292139 0.031753154 0 0.014419113 0.00681559 0.017136816 0 0.078289729 0.069626807 0.141677407 0.004816137 0.000763924 0.013546736 0 0.000780951 0 TCGA-BR-8077-01A-11R-2343-13 0.12253951 0 0.052696716 0.12253939 0 0.161597793 0.137327195 0.007511246 0.02799485 0 0 0.019861518 0.006800033 0.147859276 0.068886587 0.059867767 0.034516845 0.007349012 0.020804193 0 0 0.001848068 TCGA-FP-8210-01A-11R-2343-13 0.134541442 0.088801489 0.02533683 0.175383198 0 0.155405655 0.016935356 0.02374838 0.086869644 0 0 0.02432969 0.000804919 0.045268488 0.030414876 0.050047435 0.054349837 0.030151374 0.046845201 0 0 0.010766187 TCGA-CD-8531-01A-11R-2343-13 0 0.520117451 0.071827479 0.01254673 0 0.024288058 0.00586653 0.038140869 0.010795861 0.002882936 0.000586894 0 0 0.007466103 0 0.14215233 0 0.157877294 0 0.005451466 0 0 TCGA-CG-5721-01A-11R-1602-13 0.021003796 0.00210485 0.008919329 0.279841485 0 0.102464837 0.158028018 0.046818829 0.006783138 0 0.020976045 0.013057625 0 0.074648401 0.11332834 0.088020609 0.038143257 0 0.025861441 0 0 0 TCGA-D7-6527-01A-11R-1802-13 0.223343946 0 0.167043209 0.071842019 0 0.195855524 0.009100876 0.010266097 0.024257556 0 0 0 0.025646059 0.134266455 0.056085729 0.029851245 0 0 0.021335781 0.028311737 0 0.002793769 TCGA-VQ-A8PC-01A-11R-A39E-31 0.046330578 0.110912574 0.004420829 0.072393902 0 0.311029539 0.004909862 0 0.004289953 0 0.024796288 0 0.010834469 0 0.045779913 0.231471646 0.029086063 0.023316846 0.06248868 0 0 0.017938856 TCGA-CG-4304-01A-01R-1157-13 0.08960893 0 0.171663556 0.025680473 0 0.184093794 0.057403407 0.007505202 0.009608391 0 0.015307869 0 0 0.121758004 0.062285619 0.129998794 0.009634074 0.000854104 0.001473035 0.0051362 0.010867816 0.097120732 TCGA-HU-A4G2-01A-11R-A251-31 0.170592387 0 0.015971048 0.198559078 0 0.136896319 0.183500949 0 0.007373411 0 0 0.016849426 0 0.004436145 0.070074585 0.123000057 0.06200299 0.002306892 0.006499706 0 0 0.001937007 TCGA-VQ-A8PX-01A-12R-A414-31 0.18364134 0 0.1504592 0.188400812 0 0.258787334 0.003316401 0.000955541 0.059610933 0 0 0.02544066 0.016359226 0.014680789 0.013690666 0.003963796 0 0.041267928 0 0.015516663 0 0.023908711 TCGA-VQ-AA68-01A-11R-A414-31 0.217816065 0 0.029577192 0.140321186 0 0.152053527 0.054961619 0.016607926 0.092081709 0 0.003945171 0 0 0.085704238 0.044821904 0.070518463 0.007653466 0.017179368 0.066758167 0 0 0 TCGA-VQ-A91E-01A-31R-A414-31 0.075355787 0.006924392 0.104738611 0.185825019 0 0.318385304 0.07617226 0.039290591 0 0 0 0.016828581 0.006307674 0.045529156 0.0409925 0.05592913 0 0.007613286 0.008516402 0 0 0.011591307 TCGA-BR-7196-01A-11R-2055-13 0.183776866 0 0.121257086 0.183268166 0 0.100547149 0.050153325 0.016668209 0.014280293 0 0.039887106 0 0.024043642 0.019875979 0.109019095 0.073107114 0.017264282 0 0.04685169 0 0 0 TCGA-HU-A4H0-01A-11R-A251-31 0.056270758 0 0.014213983 0.182608836 0 0.24043961 0.154074386 0.057508204 0.002436468 0 0.000333175 0 0.00197562 0.001203217 0.139943993 0.109890986 0.005533414 0 0.033567351 0 0 0 TCGA-BR-8371-01A-11R-2343-13 0.421168929 0 0.01806632 0.09572303 0 0.190310356 0 0 0.098092944 0 0 0.055129753 0.012617329 0.011969333 0.003195397 0.034132217 0 0.021635694 0.036330962 0 0.001627735 0 TCGA-FP-7998-01A-11R-2203-13 0.073623618 0 0.035905597 0.243880692 0 0.109846562 0.126921994 0.027203324 0.016610857 0 0.036512848 0 0 0.093843565 0.082541141 0.091997119 0.00669814 0.002095416 0.052319127 0 0 0 TCGA-D7-8570-01A-11R-2343-13 0.118531654 0 0.061720096 0.083745218 0 0.168497411 0.053529258 0.051016748 0.045107186 0 0 0.032220885 0.002623864 0.13793073 0.050673498 0.103391827 0.070482828 0 0.020520584 0 0 8.21E-06 TCGA-CG-4469-01A-01R-1157-13 0.038087585 0 0.003800929 0 0 0.214153386 0.015703642 0.023454852 0 0.013832277 0.000899698 0 0 0.062289826 0.049521312 0.300638009 0.058229833 0.036321754 0 0.09683412 0 0.086232777 TCGA-BR-6455-01A-11R-1802-13 0.089129547 0 0.001458133 0.326583152 0 0.062851615 0.057821176 0.044094543 0.040639614 0 0.012798303 0 0.035093854 0.108070371 0.093568083 0.107088853 0.011516628 0 0 0.009286128 0 0 TCGA-VQ-A8P5-01A-11R-A39E-31 0.116626373 0 0.058930544 0.092587059 0 0.164877144 0.066510479 0.04056556 0.028891311 0 0.025843119 0 0 0.150607523 0.089365862 0.112563168 0.000587486 0 0.017842126 0 0 0.034202244 TCGA-CD-8534-01A-11R-2343-13 0.131484184 0 0.047233893 0.07232228 0 0.224094985 0 0 0.063889709 0 0 0.006254691 0 0.120207765 0.097855921 0.138216947 0.045829208 0 0.052610416 0 0 0 TCGA-D7-5577-01A-01R-1602-13 0.104120339 0 0.024887354 0.215598006 0 0.056222993 0.117840431 0.027375947 0.045622624 0 0.043938977 0 0.001818109 0.08013286 0.103271865 0.158798057 0.006705677 0.001848919 0.004270249 0.002532763 0 0.005014831 TCGA-BR-4257-01A-01R-1131-13 0.014911672 0 0.000424265 0.101065221 0 0.121645411 0.152628189 0.01841714 0.001701154 0 0.073685573 0 0 0.173206117 0.054446961 0.183445859 0.024070235 0 0.003309887 0 0.006730302 0.070312014 TCGA-CG-4460-01A-01R-1157-13 0.108444721 0 0.082069029 0.048266596 0 0.18899793 0.030748756 0.03289543 0.014639535 0 0.027676406 0 0 0.093306921 0.069840737 0.140713209 0.013611175 0 0 0.091301907 0.035486713 0.022000934 TCGA-BR-7901-01A-11R-2203-13 0.047986476 0 0.005200443 0.098001366 0 0.182143921 0 0.022071563 0.062010716 0 0 0.011834359 0 0.319134593 0.098556794 0.118277324 0.004609768 0 0 0.024133759 0 0.006038916 TCGA-CG-5722-01A-21R-1602-13 0.111285034 0 0.071674476 0.241552443 0 0.09732294 0.035047082 0.105839021 0.050945275 0 0 0.049393573 0.005544145 0.029165992 0.070923926 0.034334993 0.032822145 0.019121813 0.03288938 0 0.012137763 0 TCGA-BR-8295-01A-11R-2343-13 0.359219522 0 0.104697048 0.053177903 0 0.096469843 0 0.061691321 0.020039139 0 0 0.010841537 0 0.184616279 0.056690922 0.037788083 0.004830492 0 0.009937909 0 0 0 TCGA-BR-A4J7-01A-31R-A251-31 0.070872311 0 0.008714718 0.323096046 0 0.204677018 0.052917596 0.031259206 0.040282853 0 0 0.038846236 0.028285185 0.025315405 0.089473953 0.058311736 0.010398481 0 0.017549255 0 0 0 TCGA-CG-5734-01A-11R-1602-13 0.062280934 0 0.099020921 0.065142242 0 0.199569854 0.090726011 0.013371006 0.036090148 0 0.014227485 0 0 0.249273291 0.065595839 0.052060064 0.015833596 0 0.001169696 0.001007729 0 0.034631183 TCGA-VQ-A91V-01A-11R-A414-31 0.254992927 0 0.066011776 0.10928273 0 0.264244738 0 0.002818882 0.00552281 0 0.005729501 0.01993507 0.017817097 0.036440045 0.037548912 0.093574694 0 0.031153435 0.038287729 0 0 0.016639654 TCGA-FP-8209-01A-11R-2343-13 0.303700764 0 0 0.174091408 0 0.207587289 0.023706714 0 0.070116359 0 0 0.005995908 0.004675813 0.065638843 0.028273903 0.067078586 0 0.016709362 0.031026791 0 0 0.001398261 TCGA-HU-A4G8-01A-11R-A251-31 0.077669392 0.067364235 0 0.093829174 0 0.222199308 0.11480174 0.089425069 0 0 0.002148862 0 0 0.133447282 0.102749123 0.086797129 0 0.005161954 0 0 0.004406732 0 TCGA-BR-4279-01A-01R-1131-13 0.068911147 0 0.072522255 0.075994103 0 0.135691386 0.068317524 0.009595784 0 0.029868996 0.016162888 0 0.023492343 0.118195984 0.0427455 0.141724278 0.051817908 0 0.03476781 0.043143188 0.003124434 0.063924472 TCGA-D7-6522-01A-11R-1802-13 0.081192334 0.038496811 0.030586 0.147654701 0 0.126772456 0.059218008 0.021470826 0.089474988 0 0 0.001259666 0.009157781 0.102616887 0.058322066 0.162834061 0.03439327 0 0.026610532 0 0.008623263 0.001316348 TCGA-CG-4441-01A-01R-1802-13 0.11912807 0 0.010446316 0.100985064 0 0.186354392 0 0 0.05123812 0 0 9.39E-05 0 0.181714351 0.058276657 0.124213266 0 0 0 0.113262033 0 0.054287867 TCGA-HU-A4H4-01A-21R-A251-31 0.002858781 0.049476384 0.010950638 0.165752395 0 0.078796194 0.124565215 0.063537092 0.068849676 0 0.000110941 0 0 0.138892825 0.145519323 0.042443276 0 0.054298773 0.037789967 0 0 0.01615852 TCGA-VQ-AA64-01A-11R-A414-31 0.154915736 0 0.032975464 0.084175303 0 0.270146636 0 0.070944111 0.034303523 0 0 0.05478473 0.002710646 0.083310366 0.092052873 0.064469098 0 0.010165592 0.044195226 0 0 0.000850696 TCGA-D7-A6EZ-01A-11R-A31P-31 0.023895037 0 0.006186191 0.163023276 0 0.076507499 0.158790134 0.009745001 0.007845455 0 0.05816513 0 0.002208916 0.187626234 0.106028291 0.133014104 0 0.025363386 0 0.040416996 0 0.001184349 TCGA-BR-8677-01A-11R-2402-13 0.013737248 0 0 0.324323413 0 0.091998205 0.042090553 0.039704208 0.097228396 0 0 0.047720792 0.009109183 0.008298064 0.190157031 0.082986727 0.004255857 0 0.048390323 0 0 0 TCGA-RD-A8N0-01A-12R-A36D-31 0.026251884 0.206506555 0.089688361 0.177068796 0 0.202975255 0.014348033 0.077546639 0.048190606 0 0 0.018421123 0.001299372 0.016207792 0.030424423 0.031237767 0.005277077 0.009606751 0.044949566 0 0 0 TCGA-VQ-A927-01A-12R-A414-31 0.015567914 0 0.143735965 0.051010111 0 0.449303076 0.031953122 0 0.006737915 0 0.004787306 0.000177028 0.008266105 0.042731463 0.038879194 0.11711596 0.003309677 0.019087297 0.034401834 0 0.017418949 0.015517082 TCGA-RD-A7BS-01A-11R-A32D-31 0.159983014 0.05741774 0 0.090894773 0 0.192433312 0.004239343 0.031212276 0.049278196 0 0 0.023459701 0.003840749 0.140910724 0.160006619 0.023573664 0 0.013078332 0.049671555 0 0 0 TCGA-CG-5732-01A-11R-1602-13 0.034477594 0.097814391 0.083449325 0.242502501 0 0.178014951 0 0.025261536 0.089075323 0 0 0.001652946 0.009884108 0.082997857 0.03187719 0.069397798 0.002301166 0.004010868 0 0.037149845 0 0.0101326 TCGA-BR-8364-01A-11R-2343-13 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代码
setwd("") # 设置工作目录
## 读取文件，整理
data <- read.table("input.txt", sep="\t", header = T, row.names = 1, check.names = F)
data <- t(data)
col <- rainbow(nrow(data), s = 0.7, v = 0.7) # 按变量数量设置颜色。s（saturation，饱和度）和v（value，明度）用于HSV颜色空间的描述。
## 绘制
pdf("barplot1.pdf", height = 10, width = 20)
# 图形属性设置
par(las = 1, mar = c(8,5,4,16), # las用于定义轴标签的样式。mar用于设置图形的边距。
mgp = c(3,0.1,0), cex.axis = 1.5) # mgp用于设置轴标题、轴标签和轴线的边距线。cex.axis用于轴注释，相对于cex当前设置的放大倍率。
# 画条形图
a1 <- barplot(data, col = col, # 设置颜色
yaxt = "n", xaxt = "n", # 不显示x、y坐标轴
ylab = "Relative Percent", # y轴标签
cex.lab = 1.8) # 标签文字大小
# 画坐标轴
a2 <- axis(side = 2, tick = FALSE, labels = FALSE) # side用于指定要在绘图的哪一侧绘制轴。tick用于指定是否绘制刻度线和轴线。labels用于指定是否在刻度线处进行（数字）注释
axis(side = 2, a2, paste0(a2*100, "%")) # side = 2指定在绘图的左侧绘制轴
axis(side = 1, a1, labels = FALSE) # side = 1指定在绘图的下方绘制轴
# 添加坐标轴标签
par(srt = 60, xpd = TRUE) # srt表示以度为单位的字符串旋转。xpd用于设置图形打印裁剪方式
text(x = a1, y = -0.02, labels = colnames(data), adj = 1, cex = 0.6) # adj(adjustment)指定标签的x调整，0 表示左/下，1 表示右/上。cex(character expansion)，字体大小。
# 添加图例
par(srt = 0) # srt表示graphics以度为单位的字符串旋转。
ytick2 <- cumsum(data[, ncol(data)]) # cumsum(Cumulative Sums，累计总和)，将data的最后一列累计总和。
ytick1 <- c(0, ytick2[-length(ytick2)]) # 将上述总和去掉最后一位，第一位前加0
legend(x = par("usr")[2]*0.98, y = par("usr")[4],
legend = rownames(data),
col = col, pch = 15, bty = "n", cex = 1.3) # pch(point character)，点形状。bty(box type)，在图例周围绘制的框的类型。cex，字符扩展因子
dev.off() # 关闭并保存图形
